Caffeine's Effect on Hair Growth - Clincial Studies

Caffeine's Effect on Hair Growth - Clincial Studies

What is it?

Caffeine is an active ingredient found in Biotin Shampoo & Conditioner,  Spark and Spark Plus.

Scientific articles linking to growth:

Study #1:

From the International Journal of Pharmaceutical Compounding, the authors Marotta James C, Patel Gopesh, et. al have found clinical efficacy when topical caffeine is loaded at .05%.  Their conclusion was that:

"The results from this study suggest that the new hair-loss topical solution may be considered a safe and effective treatment option in male AGA patients."

For the full article, see here.

Study #2:

From the British Journal of Dermatology, the authors T.W. Fischer, E. Herczeg‐Lisztes, W. Funk, D. Zillikens, T. BĂ­rĂł, , R. Paus concluded that there are effects of caffeine on hair shaft elongation, matrix and outer root sheath keratinocyte proliferation, and transforming growth factor‐ÎČ2/insulin‐like growth factor‐1‐mediated regulation.

Their conclusion?

"This study reveals new growth‐promoting effects of caffeine on human hair follicles in subjects of both sexes at different levels (molecular, cellular and organ."

For the full article, see here.

Club Roots Products that feature this technology:

Biotin Shampoo & Conditioner, a hair growth shampoo and conditioner, contain caffeine loaded at an optimized level for better, healthier looking hair.

Spark, a topical hair regrowth treatment that blocks DHT and stimulates new hair growth in the follicle.

Spark Plus, a topical hair regrowth treatment that blocks DHT and stimulates new hair growth in the follicle.

Scientific Breakdown 

Caffeine is a nitrogenous organic compound of the alkaloid group, (1,3,7-trimethylpurine-2,6-dione) a methyl xanthine alkaloid which is consumed as a beverage, administered as a medicine or applied for cosmetic purposes. However, caffeine does not possess the properties of an ideal skin penetrant as it is a hydrophilic material with a Log P of -0.07.  Caffeine also exhibits unusual solubility behaviour in non-aqueous solvents and forms aggregates in aqueous solutions.

Effects on Hair Loss

Caffeine increases cAMP levels in cells and therefore promotes proliferation by stimulating cell metabolism; a mechanism which would counteract (DHT) induced miniaturization of the hair follicle. Encapsulated in  Niosomes, which are liposomes containing a non-ionic surfactant, and transferosomes (ultraflexible vesicles) this hydrophilic drug showed significantly greater penetration into the skin and permeation across the stratum corneum. Dose-response experiments showed that a concentration of 0.15%was stimulatory in male skin organ culture model. .High concentrations may cause an over-stimulation of hair follicle metabolism resulting in extensive consumption of energy reserves, exhaustion of the proliferation capability and finally lack of hair shaft elongation. Recent study in Identical female Twins showed less consumption of caffeine as an exogenous factor associated with increased vertex hair loss

Structure & Synthesis

Can be found naturally in tea, coffee, guarana, maté, kola nuts, and cacao. Pure caffeine (trimethylxanthine) is a white powder or could look as silky needles.Caffeine is recommended as a test substance by the OECD because it has been studied extensively in vitro and in vivo.

Mechanisms of Action

Caffeine inhibits the phosphodiesterase enzyme and has an antagonistic effect on central adenosine receptors. It is a stimulant of the central nervous system and may produce wakefulness and heightened mental activity. The molecule also increases rate and depth of respiration but it is a weaker bronchodilator than theophylline, with a  direct effect against apoptosis.

Direct Scalp Delivery

Topical delivery of active compounds to different compartments of the skin and its appendages is the prerequisite for the efficient treatment of skin diseases.The absorption of topically applied compounds and their levels in the blood vary considerably in the different regions of the human body. The density of hair follicles significantly contributes to this effect by an increase in surface area and a disruption of the epidermal barrier towards the lower parts of the hair follicle. Hereby, the human hair follicle serves not only as a reservoir, but also as a major entry point for topically applied compounds. Hair follicles are the only pathway for fast caffeine absorption during the first 20 min after application.

1. Lane Topical and transdermal delivery of caffeine. Lin Luo, Majella E. International Journal of Pharmaceutics, Volume 490, Issues 1–2, 25 July 2015, Pages 155–164
2. A Comparison of the Penetration and Permeation of Caffeine into and through Human Epidermis after Application in Various Vesicle Formulations. Skin Pharmacology and Physiology, 2016;29(1):24-30. Skin Pharmacology and Physiology can be contacted at: Karger, Allschwilerstrasse 10, Ch-4009 Basel, Switzerland. (Karger -; Skin Pharmacology and Physiology
3. Quantification of total polyphenols, catechin, caffeine, L-theanine, determination of antioxidant activity and effect on antileishmanial drugs of ethiopian tea leaves extracts. Alemu Tadesse, Ariaya Hymete, Adnan A. Bekhit, and Salahuddin Farooq Mohammed. Pharmacognosy Res. 2015 Jun; 7(Suppl 1): S7–S14.
4. Follicular penetration of topically applied caffeine via a shampoo formulation.   Otberg N, Teichmann A, Rasuljev U, Sinkgraven R, Sterry W, Lademann J.  Skin Pharmacol Physiol. 2007;20:195–8. [PubMed: 17396054]
Related Keywords: alopecia, hair loss, minoxidil, finasteride, biotin, caffeine citrate, patient satisfaction, hair growth, quality of life, anti-androgenic agent, androgens, type II 5-alpha reductase, 5AR, vitamin B, formulation, clinical study, caffeine, 
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